Test Isolated Thiobacillus T.nonellus T.versutus T.delicatus T.thioparus
Motility - - + - +
Formation of surface pellicle of sulfur particle on thiosulfate medium   + + - + +
Growth as chemolithotroph  - - - - +
Anaerobic growth and denitrification with thiosulfate - - - + -
Growth on nutrient agar + + + - -
Growth on thiosulfate agar + + + + +
Mixotrophic growth with thiosulfate +glucose + + + + -
Mixotrophic growth with thiosulfate + yeast extract + + - + -
Autotrophic growth with sulfur - - - + +
Optimum temperature 30 25-30 30-35 30-35 28oC
Optimum Ph 7 7.5-7.9 7.5-7.9 5.5-6.0 6.5-7.2
Final pH when grown on thiosulfate medium 5 5.0-5.5 5.8 -6.5 2.5-3.5 3.5-4.0
Proposed species Thiobacillus novellus
Table 2: Physiological Characteristic determined the isolated Thiobacillus strain in comparison to known Thiobacillus species (Bergy's 1989).