Figure 16: TEM photograph of E. coli treated with complex (3) showing rod cells with rough irregular cell wall (short black arrow), increased electron-lucent region between cell wall and cell membrane (long black arrow) and granular cell contents appear to be exuding from the damaged membrane (white arrow). [Specimen fixed in 4F1G solution and double stained with uranyl acetate and lead citrate, scale bar=100 nm].