Name of the test

NRB1-19

NRB1-44

NRP1-13

NRP1-14

NRP1-18

NRP1-26

NRP1-35

NRS1-11B

NRS1-18

Gram’s staining

+

+

+

+

+

+

+

+

+

Production of diffusible pigment

-

-

-

-

-

-

-

-

-

Growth at
4°C
15°C
25°C
30°C
37°C
42°C
60°C

 

-
-
+
+
+
-
-

 

-
-
+
+
+
+
-

 

-
-
+
+
+
+
-

 

-
+
+
+
+
+
-

 

-
-
+
+
+
+
-

 

-
-
+
+
+
+
-

 

-
-
+
+
+
+
-

 

-
-
+
+
+
-
-

 

-
-
+
+
+
-
-

Growth at pH range
5.2
7.0
8.0
9.0
10.0 

 

+
+
+
+
+

 

+
+
+
+
+

 

+
+
+
+
+

 

+
+
+
+
+

 

+
+
+
+
+

 

+
+
+
+
+

 

+
+
+
W
-

 

+
+
+
+
+

 

-
+
+
+
+

Growth in the presence of
2% NaCl
5% NaCl
7% NaCl
10% NaCl

 

+
+
W
-

 

+
+
+
-

 

+
+
+
-

 

+
+
+
+

 

+
+
+
-

 

+
+
+
-

 

+
+
+
W

 

+
+
+
-

 

-
-
-
-

Degradation of
Adenine 0.5%
Guanine 0.05%
Tyrosine 0.5%
Xanthine 0.4%

 

+
-
-
-

 

-
-
+
-

 

-
-
-
-

 

-
-
+
-

 

-
-
+
-

 

-
-
+
-

 

-
-
-
-

 

+
-
+
+

 

+
-
-
-

Hydrolysis of
Casein
Starch
Urea

 

+
+
-

 

+
+
-

 

+
+
+

 

-
-
-

 

+
+
+

 

+
+
+

 

-
+
-

 

+
+
+

 

+
+
-

Biochemical tests
Catalase activity
Oxidase activity
Methyl Red (MR)
Voges Proskauer (VP)
Citrate utilization
Indole production
Nitrate reduction
Gelatin liquefaction
H2S production

 

+
+
+
-
-
-
+
-
-

 

+
-
-
-
+
-
-
-
-

 

+
-
+
-
-
-
+
-
-

 

-
-
-
-
+
-
+
-
-

 

-
-
-
+
-
-
+
-
-

 

-
-
-
+
-
-
+
-
-

 

-
-
+
-
-
-
+
-
-

 

+
-
-
-
+
-
+
-
-

 

-
-
-
-
-
-
-
-
-

Table 3: Biochemical and physiological tests of the bioactive actinomycete isolates.