Enzyme |
Total vol (ml) |
Total
Protein (mg) |
Total
activity (units) |
Specific activity
Units/mg protein |
Fold purification |
Yield |
Crude |
500 |
620 |
540 |
0.89 |
1 |
100 |
Ammonium sulfate precipitation |
45 |
61.2 |
81 |
1.33 |
1.5 |
15.05 |
DEAE column chromatography |
27 |
9.45 |
36 |
3.85 |
4.33 |
6.75 |
Gel permeation |
10 |
1.73 |
23.3 |
13.5 |
15.16 |
4.31 |
|