Enzyme Total vol (ml) Total
Protein (mg)
Total
activity (units)
Specific activity
Units/mg protein
Fold purification Yield
Crude 500 620 540 0.89 1 100
Ammonium sulfate precipitation 45 61.2 81 1.33 1.5 15.05
DEAE column chromatography 27 9.45 36 3.85 4.33 6.75
Gel permeation 10 1.73 23.3 13.5 15.16 4.31
Table 2: Purification of amylase isolated from Bacillus subtilis JS 16.