Source Sum of square df Mean Square F value P value Prov>F  
Model 503.8 9 55.99 92.65 <0.0001 Significant
A - Temperature 4.84 1 4.84 8.01 0.0178  
B - pH 60.48 1 60.48 100.09 <0.0001  
C - Sugar 70.18 1 70.18 116.14 <0.0001  
AB 0.13 1 0.13 0.21 0.659  
AC 0.02 1 0.02 0.033 0.8593  
BC 49 1 49 81.09 <0.0001  
A2 173.5 1 173.5 287.1 <0.0001  
B2 91.16 1 91.16 150.86 <0.0001  
C2 115.69 1 115.69 191.44 <0.0001  
Residual 6.04 10 0.6      
Lack of Fit 4.98 5 1 4.7 0.0573 Not significant
Pure Error 1.06 5 0.21      
Cor Total 509.91 19        
Table 4: Analysis of variance for response surface quadratic model of Butanol.