RSH Medium Dry weight of biomass (g/L) Lipid content (% of dry cell weight) Lipid yield (g/L) Carbon sources RSH 12.46 ± 0.18 36.82 ± 1.43 4.59 ± 0.16 RSH1%glucose 15.39 ± 0.11 39.47 ± 1.66 6.07 ± 0.18 RSH1%sucrose 13.58 ± 0.13 38.05 ± 1.53 5.17 ± 0.17 RSH1%maltose 13.50 ± 0.12 37.71 ± 1.49 5.09 ± 0.16 Organic nitrogen source RSHLN 7.42 ± 0.11 38.89 ± 1.66 2.89 ± 0.07 RSHHN1%peptone 14.84 ± 0.19 37.91 ± 1.48 5.63 ± 0.19 RSHHN1%yeast extract 16.14 ± 0.18 38.61 ± 0.92 6.23 ± 0.18 RSHHN1%malt extract 14.25 ± 0.14 37.73 ± 1.59 5.38 ± 0.17 Inorganic nitrogen source RSHHN1%NH4NO3 13.91 ± 0.17 36.96 ± 1.61 5.14 ± 0.15 RSHHN1%(NH4)2SO4 13.28 ± 0.13 37.19 ± 1.45 4.94 ± 0.14

Table 4: Effects of supplementation of carbon and nitrogen sources on biomass yield and lipid accumulation of L. starkeyi.