a) Real time PCR for mature miR126 in HeLa, MSC and CB-EPCs. b) Quantitative fluorescence analysis of the miR126 NIR MB in microtube. Various concentrations (0, 5, 10, 20, 50 and 100 pmol) of miR126 or 100 pmol of miR126 antagomir were incubated with 50 pmol of the miR126 NIR MB. Fluorescence intensity of the miR126 NIR MB showed a positive correlation with the dose of miR126. c) Fluorescence analysis of the miR126 NIR MB in HeLa (left panel) and MSC (right panel) cells. Various concentrations (0, 5, 10 and 20) of miR126 or 100 pmol of miR126 antagomir were co-transfected into HeLa and MSCs with 50 pmol of the miR126 NIR MB. (a-c) All data were displayed as mean ± standard error (*P<0.05). d) Confocal microscopy analysis (X400) of the miR126 NIR MB in HeLa and MSC cells. Various concentrations (0, 5 and 10 pmol) of miR126 or 100 pmol of miR126 antagomir were co-transfected into HeLa and MSCs with 50 pmol of the miR126 NIR MB. Fluorescence image was acquired at excitation of 675 nm and emission of 694 nm. All figures are merged with the 4′, 6-diamidino-2-phenylindole (DAPI) image (nucleus staining, 460 nm) and cellular morphology. 10 pmol of miR126 NIR MB was transfected into HeLa cells.
