Figure 4:Neuron body detection images produced using CSIRO HCA-Vision software package. (a) The three-channel raw image, (b) Neurite channel image after smoothing, (c) Neurite image after background correction, (d) Neurite image after suppressing neuritis, (e) neurite image after thresholding, (f) segmented nucleus image in which the nuclei have been declumped and will be used as seeds for touching neuron body splitting, (g) segmented and labeled neuron bodies.