| Bacteria treatment |
Phagocytes |
Phagocytosis index (%) |
| Diurnal |
Nocturnal |
| |
|
EPEC |
S. pneumoniae |
EPEC |
S. pneumonia |
| PBS |
MN
PMN |
49.1±6.9
42.7±6.9 |
71.5±6.1#
78.5±11.8# |
48.7±4.5
45.2±5.4 |
67.8±10.3#
75.3±13.6# |
| Serum |
MN
PMN |
60.0±6.7*
59.2±8.8* |
73.2±7.8
79.0±13 |
62.3±6.3*
52.4±7.0 |
63.2±7.5
76.8±7.8# |
| Colostrum
|
MN
PMN |
62.0±5.8*
58.0±13.9 |
73.3±5.1
78.4±7.6# |
58.4±7.8*
52,3±8.9 |
70.0±5.9
70.5±7.0# |
| Purified SIgA |
MN
PMN |
58.0±4.7*
61.2±10.6 |
75.2±7.2#
81.4±4.2# |
56.6±6.9
52.1±6.4 |
71.0±2.6#
76.2±9.5# |
Bacterial phagocytosis by colostrum phagocytes was determined with the acridine orange method. Bacteria were opsonized with a normal serum pool, a colostrum
supernatant pool or purified SIgA. In controls assays, mononuclear (MN) and polimorphonuclear (PMN) cells were pre-incubated with PBS. *p=0.003 (F=5.2) comparing
the treated groups to PBS group, considering the same collection period, kind of cells and bacteria. #p=0.001 (F=14.51) comparing bacteria type, considering the same
opsonin source, kind of cells and collection time. p=0.39 (F=1.1) comparing phagocytosis between the different collection time, considering the same treatment, kind of
cells and bacteria. |
