Figure 2: Demonstration of specificity of the LAMP assay. A) Lane 1: 100bp ladder, Lane 2: negative control, Lane 3: positive control, Lane 4-11: products from LAMP reactions containing genomic DNA from bacteria which are phylogenetically similar to Orientia including R. conorii R. prowazakii, R. belli, R. rickettsii, R. typhi, R. felli, C. burnetii, and L. interrogans, respectively. B) LAMP assay performed using a combination of Orientia genomic DNA and other bacterial genomic DNA. Lane 1: 100 bp ladder, Lane 2: negative control, Lane 3: positive control, Lane 4-11: O.tsu + R. conorii, O.tsu + R. prowazakii, O.tsu + R. belli, O.tsu + R. rickettsii, O.tsu + R. typhi, O.tsu + R. felli, O.tsu + C. burnetii, and O.tsu + L. interrogans, respectively.