Hours 24 48 72 96
Treatment/ µg / ml Mean +/- SD* Growth % Mean +/- SD* Growth % Mean +/- SD* Growth % Mean +/- SD* Growth %
Control 0 ± 1.10a 100 0.005 ± 3.60a 100 0.025 ± 7.25a 100 0.011 ± 12.53a 100
0.5 0.005 ± 0.88c 80 0.028 ± 2.16c 60 0.022 ± 6.55c 90.3 0.022 ± 11.91c 95
1 0.023 ± 0.75d 68.1 0.025 ± 1.66d 46.1 0.055 ± 4.80d 66.2 0.044 ± 7.70d 61.4
1.5 0.005 ± 0.53e 68.1 0.014 ± 1.60e 44.4 0.027 ± 3.52e 48.5 0.025 ± 5.82e 46.4
2 0.022 ± 0.43f 48.1 0.021 ± 1.050f 29.1 0.014 ± 1.15f 15.8 0.011 ± 4.12f 32.8
2.5 0.006 ± 0.27g 39.0 0.01 ± 0.90g 25 0.06 ± 1.05g 14.1 0.057 ± 2.03g 16.2
*Three replicates were used for each treatment Mean and Standard Deviation were multiplied × 105
Different letters refers to presence of significant differences between treatments at P ≤ 0.05, according to Duncan’s test [13].
Table 1: Effect of different concentrations of E. arvense on numbers of Leishmania tropica. promastigotes at different time intervals.