Treatment IC50(µg/ml) Total Protein µg/ml Mean +/- SD* Total Protein % % variation Rising Reduction Control --- 2.8 ± 437.33a 100 --- --- E. arvense 1.5 0.175 ± 403.33b 92.2 --- 7.8 U. Piluifera dissolve in Cold water 1 0.005 ± 372.67c 85.2 --- 14.8 U. Piluifera dissolve in Cold and Hot water 1 0.05 ± 346.0 d 79.1 --- 20.9 * Three replicates were used f or each treatment Mean and Standard Deviation were multiplied x 105 Different letters refers to presence of significant differences between treatments at P ≤ 0.05, according to Duncan’s test [13].

Table 4: Effect of IC50 of E. arvense and U. Piluifera dissolve in Cold and Hot water on the amount of total protein (µg/ml) of L. tropica. promastigotes at log-phase (96 hrs).