| Species |
Gene Target |
Primer name |
Nucleotide sequence 5`-3` |
Working concentration (µM) |
| N .meningitidis |
ctrA |
F753
R846
Pb820i |
TGTGTTCCGCTATACGCCATT
GCCATATTCACACGATATACC AACCTTGAGCAA”T”CCATTTATCCTGACGTTCT
5`FAM;3`SpC6;”T”BHQ1 |
7.5
22.5
2.5 |
| H. influenzae |
Hpd |
HPDF729
HPDR819
PBR762i |
AGATTGGAAAGAAACACAAGAAAAAGA
CACCATCGGCATATTTAACCACT
AAACATCCAATCG”T”AATTATAGTTTACCCAATAACCC
5`HEX;3`SpC6;BHQ1 |
7.5
2.5
5 |
| S. pneumoniae |
lytA |
F373
R424
Pb400i |
ACGCAATCTAGCAGATGAAGCA
TCGTGCGTTTTAATTCCAGCT
TGCCGAAAACGC”T”TGATACAGGGAG
5`Cy5;3`SpC6;”T”BHQ2 |
5
5
5 |
