Figure 2b: Detection limit of the hly gene-based multiplex PCR. (b) Lanes: 1 and 14, 100 bp ladder (takara Bio Inc.); 2, V. Campbellii 102 CFU, 5, V. Campbellii 101 CFU, 6, V. Harveyi 104 CFU; 11, V. Parahaemolyticus 103 CFU; 12, V. Parahaemolyticus 102 CFU, 13, V. Parahaemolyticus 101 CFU. PCR products (5μl each) were analysed by 1.5% agarose gel electrophoresis and visualized after ethidium bromide staining using Gel- Doc 2000 (Bio-Rad, CA, USA).