C (mol-dm–3) C1                         f1 (mol-dm–3) C2                     f2 (mol-dm–3) E (mV) Without ergosterol With ergosterol 0.075 0.1                   0.5322 0.05                0.6402 14.5 13.6 0.15 0.2                   0.4828 0.1                  0.5322 13.1 12.3 0.30 0.4                   0.2833 0.2                  0.4828 12.8 11.4 0.60 0.8                   0.1680 0.4                  0.2833 11.9 10.1 0.75 1.0                   0.1361 0.5                  0.2441 7.5 6.4

Table 2: Membrane potential data at different mean concentration of CaCl2 with and without ergosterol at 25° ± 0.1°c.