Figure 6: Confocal microscopy images showing immunocytochemistry analysis of actin (left column, green) and myosin heavy chain (MHC) (middle column, red) expressed by muscle cells grown on chitosan-PCL randomly oriented and aligned nanofibrous scaffolds after culture in fusion media for five days. The merged images with nuclei stained with DAPI (blue) are shown on the right column. SEM images showing morphology of muscle cells grown on chitosan-PCL nanofibers after 5 days of culture. Scale bars represent 40 μm. (from ref [80] with permission).