Name Goal Lower Limit Upper Limit Importance A:BSA Is in Traget 100 mg 300 mg 3 B:Tween 80 Is in range 0.5 % 3% 3 C:Gluteraldehyde Is in range 10 % 25 % 3 Particle Size In range 104 nm 1150 nm 3 Entrapment Efficiency Maximise 33 % 78 % 3

Solution No. BSA Tween 80 Gluteraldehyde Particle Size Entrapment Efficiency Desirability 1 100.00 3.00 25.00 660.12 79.12 1 2 102.01 2.99 24.64 664.22 78.19 1 3 103.34 2.81 24.95 616.43 78.15 1 4 120.69 2.56 19.69 540.96 74.69 1 5 115.14 2.98 24.98 614.79 78.01 1 6 104.38 3.00 24.74 656.05 78.25 1 7 157.69 2.76 21.39 430.69 71.69 1 8 103.99 2.98 24.65 657.11 78.05 1 9 100.50 2.82 24.93 624.74 78.31 1 10 112.32 2.99 24.89 628.29 78.05 1

Table 4a: Set parameters and some of the suggested solutions of PTX-BSA nanoparticles.