Solution No./ Run BSA Tween 80 Gluteraldehyde Particle Size Entrapment Efficiency Predicted response (%) Obtained response (%) Predicted response (%) Obtained response (%) 1/B9 100.00 3.00 25.00 660.12 657.69 79.12 78.69 4/B10 120.69 2.56 19.69 540.96 549.69 74.69 75.65 7/B11 157.69 2.76 21.39 430.69 438.65 71.69 70.65

Table 5a: Predicted response and related obtained response of three of selected formulation of PTX-BSA nanoparticles.