Isolated Bacteria/Yeasts 0 hrs 24 hr 48 hr A B C D A B C D A B C D Acetobacter aceti - - - - - + - - - + - + Enterobacter sp - - - + - + - + - + - + Micrococcus sp - + - + - + - + - + - + Leuconostoc sp - + - + - - + + - - + + Lactobacillus sp - + - + - + + + - + - + Bacillus sp - + - + - - - + - - - + Staphylococcus sp - - - + - - - + + + - + Saccharomyces cerevesiae + - - + + + - + + + - + Saccharomyces bailii + - - + + + - + + - - + Saccharomyces chevavieri + - - + + - - + + - - + Candida tropicalis - - - + + - - + + - - + A-Samples treated with sodium metabisulfite; B- sodium benzoate; C- calcium hyroxide; D – control ; (+): Presence; (-): Absence.

Table 3: Microorganisms isolated from the toddy collected with food grade preservatives.