Figure 3: Impact of IGF-1 addition on exercise-induced expression of IGF-1 receptor and PI3K in mouse skin tissues. SENCAR mice were exercised with an iso-caloric diet for 12 weeks. IGF-I was injected at 10 μg/g B.W. twice per week in the last two weeks. The levels of IGF-1 receptor and PI3K protein in skin tissues was determined by Western blotting and quantified by the FluorChem 8800 Advanced Imaging System. No statistical difference of the levels of IGF-1 receptor between treatment groups was found. The levels of PI3K expression was significantly decreased in PE group, but that reduction was reversed by IGF-1 addition (means with different letters differ significantly, P < 0.05, n = 5-7.