Figure 7: Expression level of AIF protein and representative confocal image for AIF translocation into the nuclei of AEL-treated MCF-7 cells.MCF-7 cells were treated with 50 and 100 μg/mL AEL for 48 hrs. (A) Cell fractions were isolated and analyzed by Western blotting using anti-AIF antibody. (B) Subcellular localization of AIF was analyzed by immune cytochemistry using antibody against AIF.