Replicate 1 VF Coverage Chromosome Position Reference Mutation Replicate 2 VF Yes=2nd file within the range Replicate 3 VF Yes=3rd file within the range *Yes in Replicates 2 & 3
0.0105 956 18 48603079 C T         N/A
0.0131 915 18 48603083 G A         N/A
0.0118 847 7 55221909 G A         N/A
0.0543 847 7 55221915 T G 4.65% Y 5.34% Y Y
0.0201 845 7 55221903 C T         N/A
0.0195 822 7 55221878 C T         N/A
0.0122 817 20 36031726 C T         N/A
0.1077 808 20 36031767 C G 13.50% Y 11.38% Y Y
0.0137 805 20 36031725 C T         N/A
0.015 802 7 55221852 C T         N/A
0.055 782 7 55221914 C T 6.43% Y 6.81% Y Y
Datawithcoveragebetween782and322werehiddento condensethetable  
0.0373 322 19 17945621 G A         N/A
0.0374 321 2 212652721 G A         N/A
0.0596 319 19 1207074 G A #N/A #N/A 4.22% Y #N/A
0.0314 318 4 55979610 G A         N/A
0.0411 316 9 5073849 A T         N/A
0.1206 315 19 1207082 A G 9.32% Y 7.24% Y Y
0.0351 313 7 116411973 C T         N/A
0.0321 312 13 48942631 G A         N/A
0.0452 310 15 90631878   G A         N/A
0.0356 309 7 128851504 C T         N/A
0.0552 308 7 128851501 A G 3.28% Y 4.55% Y Y
0.0525 305 7 128851502 G A 2.70% Y 4.86% Y Y
0.1711 304 19 1207084 G A 13.95% Y 8.07% N N
0.1419 303 20 57484567 C T 12.63% Y 12.00% Y Y
0.3709 302 4 153249358 A T         N/A
0.0464 302 7 128851500 G T         N/A
0.04 300 17 7579879 G A         N/A
*N/A=replicate1VF>25%(0.25)
N=oneNandoneY,or twoNsforthetworeplicates
#N/A=oneormore#N/Awereidentifiedfromthetworeplicates
Table 9: Effect of applying a more stringent filter to same data set as in Table 8 (Ion Torrent Intra Run data).