SampleID Chromosome Position Reference Mutation Variant Frequency Coverage Platform Sequnome Variant Call Sequnome VF CRC80 4 55152040 C T 0.143 5138 Miseq IonTorrent T 0.35 4 55152040 C T 0.306 421 CRC83 10 43613843 G T 0.090 222 Miseq IonTorrent T 0.42 10 43613843 G T 0.239 654 CRC86 22 24134064 C T 0.087 2705 Miseq IonTorrent T 0.05 22 24134064 C A 0.074 558 CRC88 5 112175571 C A 0.0866 3073 Miseq IonTorrent T 0.07 5 112175571 C T 0.0939 181 CRC89 17 7578253 C A 0.1201 6204 Miseq IonTorrent A 0.35 17 7578253 C A 0.3196 388 CRC89 18 48581234 C T 0.1706 3604 Miseq IonTorrent T 0.15 18 48581234 C T 0.0823 486 CRC94 10 89717672 C T 0.897 1563 Miseq IonTorrent T 0.61 10 89717672 C T 0.5513 390

Table 11: List of discordant calls with tie-breaker Sequenom assay results.