| Gene name |
Amplicon (bp) |
Sense |
Sequence (5'to3') |
Length (bp) |
Tm |
| TRAP |
162 |
F |
CGGAGAGGTAACCAAGAACAAG |
22 |
60.2 |
| R |
TGTGACCGTCTATTTCGTCATC |
22 |
60 |
| Osteocalcin |
119 |
F |
TCTCTCGCTCTCCCTCTAACAC |
22 |
60.2 |
| R |
TCTATTCTGCAAGGCTTTTTGG |
22 |
60.6 |
| Mef2c |
158 |
F |
ACCCACAAATACACAACACAGG |
22 |
59.7 |
| R |
GTCTGAAATGGAACCCTATTGC |
22 |
59.8 |
| β-actin |
133 |
F |
GAAAGACAGCTACGTGGGAGAC |
22 |
60.2 |
| R |
CTCGTTGTAGAAGGTGTGATGC |
22 |
59.8 |
| Heat shock protein 90 B |
232 |
F |
CGACTTCATCAAGCTGTTTGTC |
22 |
59.9 |
| R |
CTTGTCGTTGTACTGCTCTTCG |
22 |
60.1 |
|
