|
Phenotypes |
Sirius red (basalias) |
Von Kossa (trabecular bone) |
mCT |
| Non mineralized area (%) |
Intermediary mineralized area (%) |
Mineralized area (%) |
Total area (mm2) |
Mineralized area (mm2) |
Osteoid (mm2) |
Tt.B.Ar (%) |
| Normal |
59.4 ± 11.5 |
32.0 ± 9.6 |
8.5 ± 1.9 |
5931.2 ± 249.8 |
5711.0 ± 233.1 |
220.1 ± 16.7 |
39.3 ± 3.2 |
| Deformed |
68.6 ± 7.8 |
17.7 ± 2.0 |
13.7 ± 6.0 |
5442.0 ± 110.1 |
5166.1 ± 99.8 |
275.9 ± 10.7 |
43.3 ± 3.6 |
|
| Phenotypes |
Fork length (cm) |
Weight |
Condition |
Stiff. |
P Vt. |
Ash Vt. |
P Sc. |
Ash Sc. |
P plasma |
| (g) |
factor (K) |
(N/mm) |
(%) |
(%) |
(%) |
(%) |
(mg/dl) |
| Normal |
35.1± 0.6 |
657.3± 33.2 |
1.5 ± 0.0 |
140.2 ±6.3* |
9.3 ± 0.3 |
59.4 ± 0.5* |
5.5 ± 0.1 |
29.8 ±0.7* |
11.5 ± 0.4* |
| Deformed |
32.6 ± 0.8 |
526.9 ± 49.6 |
1,6 ± 0.1 |
80.3 ± 9.0* |
8.8± 0.3 |
55.0 ± 0.4* |
4.4 ± 0.5 |
26.3 ±1.1* |
7.6 ± 0.7* |
|
| Table 3: Growth, condition factors, stiffness, P mineralization status (dry tissues) calculated as ash or P content and results of histological observations by Sirius red and
Von Kossa. Values are expressed as means (n=3) ± sem. P: Phosphorus; Sc: Scales; Vt: Vertebrae; Tt.B.Ar : Total Bone Area. Asterisks (*) represent significant difference
(P<0.05). |
