Sequencing Normal Deformed
Ave. number reads 44.6 M 46.1 M
Ave. number trimmed reads 35.3 M 37.8 M
Ave. mapping (%)* 71.7 71.6
DE analysis Def. Vs. Norm.
Up-regulated in Def. 700
Down-regulated in Def. 589
Total 1 289
Match Uniprot 793
FDR: 5%, triplicates. Unique gene match in Uniprot-Swissprot database (cut-off evalue<1e-8). Analyses on genes levels expression with both edgeR and DESeq packages.
*Trimmed reads with at least one reported alignment using RSEM output.
Table 4: Sequencing results of biconcave (Deformed, Def.) and normal (Norm.) vertebra. DE: Differentially Expressed.