Figure 1: Single-molecule detection platform, including temperature control system and flow delivery system, was integrated into an inverted fluorescence microscope (upper left). The lower right figure showed the schematic of streptavidin-conjugated quantum dots with biotinylated ligand EGF, where binding of EGF to the extracellular domain of EGFR leads to receptor dimerization and tyrosine autophosphorylation of receptor tyrosine kinases.