Figure 1: Specific FD inhibit human MC calcium-ionophore and poly-L-lysine-induced mediator release in a dose dependent manner. Mast cells were incubated overnight with FD, washed and activated with increasing concentrations of A23187 (A and B) or poly-L-lysine (C and D) for 30 minutes (degranulation, A and C) or 18 hours (cytokine, B and D). Mediator release was assessed as described in materials and methods. Figures are representative of three separate MC cultures with each condition performed in triplicate.