Gene Expression NM 300 NRCWE 002 IL6 0 0 IL10 +200% +200% C3 -100% -100% CXCL2 0 0 TNF-α +50% 0 Fas Ligand 0 0 ICAM-1 -100% -100% Albumin 0 0

Table 3: mRNA expression of inflammatory cytokines and receptors, FasL and albumin in C57/BL6 mice liver. The animals were exposed three times (0 hr, 24 hr and 48 hr ) to 100 μl of 64 μg/ml of the Ag (NM 300) and the positively charged TiO2 NMs by injection via the lateral tail vein. The animals were dissected 72 hr after the first injection and the mRNA expression was analysed by real time PCR, with B2m utilised as an endogenous control.