Figure 4: Inhibition of melanoma tumors in an additive manner following treatment with a nanoparticle containing siRNAs directed against two different targets. (4A) SiMutB-Raf and siAkt3 cooperate to reduce anchorage independent growth in cell culture. (4B) SiAkt3 and siMutB-Raf act additively to inhibit cell viability. Calculation of the CI index for the combination of siAkt3 and siMutB-Raf showed additive inhibition of cell viability with CI values between 0.94 and 1.10. (4C) Ultrasound treatment followed by topical application of siAkt3-liposomal complex containingsiMutB-Rafdecreased melanoma development in animal skin.