Figure 1: Nano-volume well chip for high-throughput analysis of single cells. Microscopic images with low (A) and high (B) magnification of the fluorescently labeled nano-volume well-array chip are shown. Optical microscopic image of the nano-volume well taken from the top following attachment of one single mCherry+ cancer cell (C). Transversal confocal microscopic image of a well (D). The green lines result from laser light reflection when encountering the different parts of the chip, e.g. top of the well, bottom of the well, and bottom of the chip. One mCherry+ cell is attached to the well (E) Table describing the chemical groups present on the chip surface after treating the chip with the different coatings (left). Optical images of nano-volume wells treated with different coatings (right), from top to bottom: untreated, treated with collagen, and treated with ULA.