Figure 3: Manipulation and phenotyping of expanded cultures. Spheres generated after 7 days of cultivating primary pancreatic cancer cells (expressing GFP as a reporter gene) at the single-level using the nanovolume well chip (A, upper panel). The same nano-volume well chip after a single sphere had been picked by a XYZ-axe arm from one of the nanovolume wells, highlighted as a red circle (A, lower panel). Sphere colony after being picked from the nano-volume well (B, upper panel), and the same sphere colony after being expanded for two and four weeks in a 96-well plate (B, lower panel). Fluorescence and bright-field images are shown. Immunostaining of a sphere (C) and cell monolayer (D) derived from primary pancreatic tumor cells. Dapi stained the cell nucleous (blue) and EpCAMAF488 stained the cellular membrane (green).