Figure 5: (a) Biogenic SeNPs decrease the viability of PC3 cancer cells. PC3 cells were cultured in the presence of SeNPs or SeMet at 0-6 μg Se/mL concentration for 24 h. Cell viability was quantified by XTT assay. Results were verified by three repetitions of experiments and each experiment was conducted in triplicate. *p < 0. 01 represents significant difference in the viability of PC3 cells treated with SeNP or SeMet. The difference in the cell viability between SeNP concentrations of 2 μg Se/mL, 3 μg Se/mL, and 4 μg Se/mL with respect to 1 μg Se/mL were also statically significant with p value < 0. 01. (b) Bright field microscopy at a magnification of 40 X representing the morphological responses in PC3 cancer cells after 24 h of treatment with SeNP or SeMet at 0-6 μg Se/mL concentration.