Figure 6: Biogenic SeNPs induced necrosis in PC3 cells but failed to induce apoptosis or necrosis in hPBMC. (a) PC3 cells (b) and hPBMC were cultured in the presence of SeNPs or SeMet at a concentration of 2 μg Se/mL for 24 h, while the control cells were not treated. The percent of apoptosis/necrosis was measured by Annexin-V and PI staining. Data presented are representative of flowcytometric experiments conducted in triplicate. PC3 cells (c) and hPBMC (d) were cultured in the presence of SeNPs at a concentration of 2 μg Se/mL for 12 h. Caspase-3/7 activity was measured by using ApoTox-Glo assay kit containing a luminogenic caspase-3/7 substrate. Cleavage of this substrate by caspases produced a “glow- type” luminescent signal. The amount of luminescence is proportional to the activity of caspases in the cells. Experiment was conducted in triplicate. *p<0. 01 represents significant difference in the level of caspase-3/7 activity between SeNP treated and untreated PC3 cells