Figure 5: Cell viability assessment by alamarBlue® shows reduction in viability with different concentrations (0, 25, 50 and 100 μg/mL) of synthesized Ag NPs treated promastigotes (a) Graph shows that the synthesized Ag NPs were most active against promastigotes of L. donovani compared to aqueous leaves extract, AgNO3 and TiO(OH)2 solutions and synthesized TiO2 NPs (b) Microscopic images of wild type promastigotes and promastigotes treated with aqueous leaves extract of E. prostrata, AgNO3 solution and synthesized Ag NPs at a magnification of 60X (c) Percentage growth inhibition against log value of synthesized Ag NPs concentrations (μg/mL), IC50 = 14.94 μg/mL (d) Percentage growth inhibition of L. donovani promastigotes treated with synthesized Ag NPs concentrations. The data are presented as means ± standard deviation of three independent experiments.