Figure 8: Synthesized Ag NPs induced cell cycle arrest in the G0/G1 phase of L. donovani parasites. After treatment with different concentrations of synthesized Ag NPs (a, b, c and d) for 24 h, promastigotes were collected, washed with PBS and stained with PI, the DNA content was analysed by flow cytometry (e) Graph showing % population in G0/G1, S and G2/M phase of parasites treated with different concentrations (12.5, 25 and 50 μg/mL) of synthesized Ag NPs.