Figure 4: Time course of changes in nuclear CHOP (A) and C/EBPβ (B) expression in rats following intraperitoneal administration of Ag-nps. Upper panels of part A and part B: Nuclear CHOP and C/EBPβ proteins expression were analyzed by Western blot analysis. Middle panels of part A and part B: Coomassie blue-stained gels demonstrating equal loading of the samples. Molecular weight size markers (kDa) are indicated at the left of each image. Lower panels of part A and part B: The relative densities were normalized from upper panel to the total amount of protein measured from middle panel. Nuclear protein fractions were processed for Western blotting as described under Materials and Methods. Values were means ± SD (n=7).