Figure 9:pDNA delivery in HSkM cell line transfected with polymer/pDNA complexes at weight ratios of 5 &10 after 48 hrs incubation. [T85 (10 µg),Z1-Z9 (5/10 µg), PEI 25k (5 µg); pDNA 1 µg in 500 µl medium in HSkM cell line. Column A: GFP expressed illustrated under fluorescent microscopy; Column B and C illustrate living cell percent of collected cells and GFP expression, respectively. D) Transfection efficiency analyzed with FACS (*p<0.05 compared with PEI 25k, two-tailed Student’s t-test, error bar represents standard deviation, n=3). E) Cell viability (*p<0.05 compared with untreated cell as control, two-tailed Student’s t-test, error bar represents standard deviation, n=3).