Figure 1: Effects of treatment with silica particles on neutrophil levels in mice. BALB/c mice were intravenously injected with nSP70 (0.8 mg/mouse), nSP300 (0.8 mg/mouse), mSP1000 (0.8 mg/mouse), or saline. BALB/c mice with no treatment were divided into untreated group. (A) The proportion of neutrophils in peripheral blood of each mouse was determined by flow cytometry 24 h after treatment. (B) The neutrophil count in peripheral blood of each mouse was calculated by multiplying neutrophil proportion by white blood cell count. (C) The effect of nSP70 dose (0.2, 0.4, or 0.8 mg/mouse) on the proportion of neutrophils in peripheral blood of each mouse was determined by flow cytometry 24 h after treatment. (D, E) The proportion of neutrophils in peripheral blood of each mouse was determined by flow cytometry 2 h (D) and 72 h (E) after treatment. Data are presented as mean ± SEM; n = 5–6; **P < 0.01 vs saline-treated group.