Protein

Film pressure (mN/m)

Protein concentration in film (mg/ml)

Spreaded volume (μl)

Protein solution in drop (μl)

Reservoir solution in drop (μl)

Reservoir solution in well (ml)

T
(°C)

 Number of layers

Ribonuclease A

20

5

100

3

3

1

20

20

Proteinase K

20

10

100

3

3

1

20

20

Thaumatin

20

1

50

3

3

1

20

20