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Figure 7: Analysis of uptake mechanisms using different inhibitors in A549 cells in the presence of serum. Cells were pretreated with various inhibitors, Genistein, Filipin and Chlorpromazine, for 30 min, followed by 2h exposure to SiO2 NP. Given are means ± SEM of 2 independent experiments, each with 3 replicates. The normalized fluorescence (% uptake) were calculated by dividing the SiO2 NP fluoresence in the presence of inhibitor by the fluorescence of controls without inhibitors. |