Figure 7: In vitro SERS and fluorescence mapping results following 3 hour treatment with NS1 on SCC9 cells. Figure 7 shows the brightfield image (A), corresponding cell fluorescence image (B) and SERS image (C) demonstrating the expression and relative distribution of NS1 in SCC9 cells. Fluorescence image was achieved at 532 nm excitation. Raman intensity images were created using the sum filter from the 1398-1447 cm-1 peaks of DTTCi at 785 nm laser excitation.