Table 1

Variables			Code	Levels
Variables			Code	1	2
Cross Linking agent			X1	0.5 gm	1gm
Drug			X2	20 mg	50 mg
Formula code	X1	X2	Responses
Formula code	X1	X2	Particle diameter	PDI	Zeta Potential	Entrapment efficiency (%)^a
F1	0.5	20	32.67	0.519	-29.1	63.72
F2	0.5	50	24.36	0.537	-19.5	78.98
F3	1	20	68.06	0.385	-7	54.05
F4	1	50	141.8	0.365	-5.15	43.36

^aAverage of three experiments

Table 1: Experimental condition, design and responses of 2² factorial design preparation of IND nanoparticles.