Response Variable Sum of squares dfa F value Prob>F Particle Diameter X1 5839.2500 1b     X2 1070.2712 1     X1*X2 1070.2712 1     PDI X1 0.0234 1     X2 0.0000 1     X1*X2 0.00361 1     Zeta Potential X1 332.15 1     X2 32.77 1     X1*X2 15.02 1     Entrapment efficiency (%) X1 512.796 1     X2 5.220 1     X1*X2 168.350 1     adegrees of freedom. bSignificance level based on 1 df; p<0.01. Best formulation selected by the program JMP is X1; 0.5 and X2; 20 Desirability; 0.66094

Table 3: Analysis of variance (ANOVA) for a 22 Factorial design experiment for evaluation of variables’ effect on the IND formulations.