Response Variable Sum of squares dfa F value Prob>F Particle Diameter X1 3.705 1b     X2 112.254 1     X1*X2 3.706 1     PDI X1 0.0136 1     X2 0.00462 1     X1*X2 0.04928 1     Zeta Potential X1 222.457 1     X2 107.848 1     X1*X2 177.289 1     Entrapment efficiency (%) X1 247.747 1     X2 90.250 1     X1*X2 1.78 1     adegrees of freedom. bSignificance level based on 1 df; p<0.01. Best formulation selected by the program JMP is X1; 0.5 g and X2; 50 mg. Desirability; 0.6480

Table 4: Analysis of variance (ANOVA) for a 22 Factorial design experiment for evaluation of variables’ effect on the ACV formulations.