Figure 3:Effect of lipid nanoparticle constituents, presence of fluorescent dye and production parameters on particle dimensions, as determined by photon correlation spectroscopy analysis. Graphs show the Z-average (A) and dispersity (B) (± SD, n=5) of lipid nanoparticles constituted of solid lipids (plain bars), a mixture of solid and liquid lipids (striped bars) or solid lipids plus the fluorescent dye cardiogreen (green bars).After production, lipid nanoparticle were incubated at 40°C, for 30 min, in the absence (light bars) or in the presence of polysorbate 80 (dark bars). For nanoparticle identification codes, composition and preparation procedure, please refer to Table 1 and experimental section.