Figure 5 :Effect of aging on the morphological (A-C) and dimensional (D) characteristics of lipid nanoparticles. Cryo-TEM photomicrographs were taken after 90 days from the preparation of #SLN(A), #SLN-P80 (B) and #SLN-P80-CG (C). Panel D shows the variation of Z-average (± SD, n=5) within the indicated length of time for #SLN (open circles), #SLN-P80 (filled circles) and #SLNP80- CG (filled squares), data are the mean of 3 determinations on different nanoparticle batches. For nanoparticle identification codes, composition and preparation procedure, please refer to Table 1 and experimental section.