Figure 3: Stability of polyplexes in the presence of heparin. Polyplexes containing PEA complexed with AO were incubated with varying amounts of heparin prior to separation on agarose gels. All polyplexes contained 1 μg of AO, and were prepared at the same weight ratio of polymer/AO = 5. The full range of heparin concentrations examined (100, 50, 25, 10, 0 μg/mL from left to right for each sample).