Figure 4: A) Cy3 labeled 2′-OMePS (Cy3-Oligo) with and without polymer formulation in C2C12 cell line. B) Representative fluorescence images of 2′-OMePSE50 -induced exon-skipping in C2C12E50 cell line. C) Cell morphology under bright field. 2′-OMePSE50 (2 μg) formulated with PEA polymers (10 μg), PEI 25k (2 μg) in 0.5 mL 10% FBS-DMEM. The images were taken 48h after treatment. Original magnification, x100. D) Statistical date of TE of 2′-OMePSE50 (2 μg) formulated with polymers (n = 3, two-tailed t-test, *p ≤ 0.05 compared with 2 μg 2′-OMePS only). E) Cell viability with 2′-OMePSE50. The results are presented as the mean ± SD in triplicate (n = 3, two-tailed t-test, *p ≤ 0.05 compared with untreated cell as control).