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Figure 6: Microscopic images obtained in (A) bright field and by (B) afluorescence microscope of the mixture (1 mL) containing MCF-10A cells (~40000 cells) and breast cancer cells Hs578T (~40000 cells) obtained after incubated with Glu-Fe3O4 (~500μg) for 4 h followed by rinse with PBS and stained with Hoechst 33342 dye. Microscopic images obtained in (C) bright field and (D) by a fluorescence microscope obtained from the mixture (1 mL) containing MCF-10A cells (~40000 cells) and breast cancer cells Hs578T (~40000 cells) after incubated with bare Fe3O4 (~500 μg) for 4 h followed by rinse with PBS and stained with Hoechst 33342 dye. The excitation wavelength was set at 330-380 nm and the emission wavelength was set at 420 nm. The scale bar is 10 μm. |